The Genome Engineering Core Facility

The Genome Engineering Core Facility
(Formerly the BAC-Recombineering Core Facility)
Jennifer R. Moran, David Steffen, and Kevin P. White

The past few years have seen an explosion in genome engineering techniques, making possible experimental design on a new, more ambitious scale. Inexpensive in vitro DNA synthesis can be combined with large-molecule manipulation methods such as BAC-Recombineering, and with genome editing methods such as CRISPR, to generate an unlimited variety of cell lines and transgenic organisms for specific experimental applications.

The Genome Engineering Core Facility performs a variety of recombination-mediated molecular cloning techniques for IGSB members and the larger scientific community. Recombineering projects include transcriptional and translational reporters, deletion of regulatory elements, site-directed mutagenesis, and other molecular genetic manipulations. We produce GFP-tagged BACs on a high-throughput scale for the ENCODE consortium. We continue to incorporate new methods, such as Gibson Assembly, Polymerase Chain Assembly, and now CRISPR, to meet the research needs of the research community. We provide pre-experimental consultation to investigators who seek to incorporate advanced transgenic technology into their research programs.

Contact Information

Questions relating to the GEC services or to BAC-recombineering and CRISPR in general should be directed to .(JavaScript must be enabled to view this email address), Scientific Director.

 

Genome Engineering Team:
• Jennifer Moran, PhD Scientific Director
• David Steffen, Research Technician

IGSB Genome Engineering Core Facility (GEC)
Knapp Center for Biomedical Discovery, 10th Floor
900 E. 57th Street
Chicago, IL 60637
Phone: 773-834-0074
Fax: 773-834-2877